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Quick Search     Adv Search 2023 Vol. 37, No. 3 Published: 2023-03-31 Reviews Basic Research Analysis & Detection        Basic Research 1 Isolation, Identification and Spoilage Ability of Dominant Spoilage Bacteria from Channel Catfish GAO Tianqi, WANG Chao, WANG Lan, SHI Liu, CHEN Sheng, WU Wenjin, CHEN Lang, XIONG Guangquan DOI: 10.7506/rlyj1001-8123-20230110-005 This study was conducted in order to investigate the specific spoilage organisms (SSOs) in cold stored channel catfish and their spoilage ability. Eight strains of spoilage bacteria were isolated and identified from channel catfish at the end of refrigerated storage by traditional selective culture combined with 16S rDNA sequence analysis. Based on the sensory score of channel catfish inoculated with each of the spoilage strains after 0–8 days of storage, four strains of SSOs were selected for identification by their colony and morphology characteristics, physiological and biochemical properties and 16S rDNA sequence. The strains were inoculated into sterile fish fillets and stored at 4 ℃ for up to eight days. The total number of colonies and total volatile basic nitrogen (TVB-N) content were determined during the storage period. The yield factor of spoilage bacteria metabolites (YTVB-N) was used as an indicator to measure the spoilage ability of spoilage bacteria. The results showed that the SSOs in channel catfish were two strains of Shewanella putrefaciens, Pseudomonas aeruginosa and Pseudomonas aeruginosa. All the four strains could cause spoilage of sterile fish fillets, with Shewanella having the strongest spoilage ability. 2023 Vol. 37 (3): 1-6 [Abstract] ( 172 ) 全文 ( 289 )        Analysis & Detection 7 Analysis of Animal Derived Ingredients in Meat Products by using Optical Gene Thin-Film Biosensor Chips and Real-Time Fluorescence Quantitative Polymerase Chain Reaction (RT-qPCR) SHAO Biao, GAO Liting, XU Chenhong, ZHANG Xia, CHEN Gang, WANG Shaoyun DOI: 10.7506/rlyj1001-8123-20221027-143 For the purse of investigating animal derived ingredients in meat products in order to help in identifying adulteration in meat products, optical gene thin-film biosensor chip technology was used to screen and analyze the animal derived components of 23 samples of meat floss, sausage, meat roll, pre-processed meat and dried meat sold on the market. The obtained results were consistent with those of real-time fluorescent quantitative polymerase chain reaction (RT-qPCR). The biosensor chip enabled more efficient screening of unknown samples. When random samples were analyzed using the biosensor chip, the results for 21.7% of these samples were inconsistent with the label information, so adulteration and false labeling of meat products should not be ignored. 2023 Vol. 37 (3): 7-13 [Abstract] ( 123 ) 全文 ( 55 ) 14 Proficiency Testing for Determination of Nitrite in Meat Products BU Dandan, YU Libo, BAI Yaxin, YANG Zhaoying, DONG Jing, YU Yang, CHEN Dongdong, PENG Tao DOI: 10.7506/rlyj1001-8123-20230110-005 To strengthen the safety supervision and management of meat products, further improve the technical ability of inspection and testing institutions to detect nitrite content in meat products, and ensure the provision of accurate and reliable testing data, this work organized and implemented a proficiency testing for the determination of nitrite in meat products. Chicken sausage containing sodium nitrite was prepared and randomly assigned to 42 participating laboratories for nitrite detection. The laboratories were required to return their results for robust statistical analysis. The testing ability of these laboratories was evaluated by Z scores and technical analysis was carried out on the laboratories that provided non-satisfactory results based on the original records. The results showed that the prepared samples were homogeneous and stable, which could meet the requirements of the proficiency testing program. The overall satisfaction rate of the 42 laboratories was 81.0%, indicating that they had a good ability to detect the content of nitrite in meat products. The problems of some of the laboratories should be corrected to improve their testing ability. 2023 Vol. 37 (3): 14-20 [Abstract] ( 120 ) 全文 ( 161 ) 21 Determination of Adrenergic Drug Residues in Pork by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry LIU Yongjun, LI Ting, LIU Hongbin, LEI Chunjuan DOI: 10.7506/rlyj1001-8123-20220929-130 An analytical method for simultaneous determination of adrenergic drug residues in pork was developed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). The instrument was operated using an electrospray ionization source in the positive ion mode with multiple reaction monitoring (MRM). Qualitative and quantitative analysis was performed by the internal standard method. The limit of quantitation was 5 μg/kg for isoprenaline, phenylephrine and metaraminol; 10 μg/kg for adrenaline and dopamine; and 50 μg/kg for noradrenaline. The calibration curves were linear in the concentration range of 1.0–100.0 μg/L with correlation coefficients (R2) greater than 0.99. The average recoveries of spiked samples were in the range of 81.9%–93.1%. The proposed method is simple, rapid, reliable, stable, highly sensitive, and suitable for large-scale laboratory analysis of adrenergic drug residues in pork. 2023 Vol. 37 (3): 21-27 [Abstract] ( 183 ) 全文 ( 1033 ) 28 Development of Proofman-Ladder-Shape Melting Temperature Isothermal Amplification Method for Detection of Pig-Derived Components in Meat Products WANG Borui, XU Dandan, GU Menglin, YAO Wei, WANG Yao, XIAO Fugang, WANG Deguo DOI: 10.7506/rlyj1001-8123-20221215-156 The study aimed to establish a proofreading enzyme-mediated probe cleavage combined with ladder-shape melting temperature isothermal amplification (Proofman-LMTIA) method for detection of pig-derived components in meat products. The LMTIA primers and Proofman probes were designed using the porcine prolactin receptor (PRLR) gene as the target. The LMTIA reaction system was optimized, and the specificity, sensitivity and limit of detection (LOD) of the proposed method were determined. The results showed that the amplification could be completed within 30 min, and the Proofman-LMTIA method could specifically detect the genomic DNA of pigs rather than of chicken, ducks, cattle, sheep, dogs, sweet potato, cassava, mung bean and wheat. The sensitivity was 1 ng/μL, and the LOD for artificially adulterated meat samples was 0.1%. The Proofman-LMTIA method has high specificity for pig-derived components, and can quickly and accurately detect pig-derived components in meat products and rapidly detect meat adulteration. 2023 Vol. 37 (3): 28-32 [Abstract] ( 140 ) 全文 ( 925 ) 33 Determination of Eleven N-Nitrosamines in Animal Derived Foods by Gas Chromatography-Tandem Mass Spectrometry after Pass-Through Solid Phase Extraction WANG Yanli, LIANG Xiuqing, CHEN Qianqian, LI Fangfang, LI Jie, CHEN Keyun, TIAN Qiyan, LI Xia, LIU Yanming DOI: 10.7506/rlyj1001-8123-20230110-003 A method was developed for the determination of 11 N-nitrosamines in animal derived foods by pass-through solid phase extraction coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS). The rehydrated samples were extracted with acetonitrile and rapidly purified by using a Prime HLB solid phase extraction column (200 mg/6 mL). The target compounds were detected in the multiple reaction monitoring (MRM) mode, and quantified by the matrix matched external standard method. The calibration curves for the 11 N-nitrosamines were linear in the concentration range of 0.5–500.0 ng/mL, with correlation coefficients of 0.999 4–0.999 8. The limits of detection (LODs) and the limits of quantitation (LOQs) were 0.2–0.3 and 0.5–1.0 μg/kg, respectively. The average recoveries at spiked levels of 1, 4 and 10 μg/kg ranged from 80.1% to 97.4%, and the relative standard deviations (RSDs) were between 2.3% and 8.8%. The extraction and purification time required by this method was only 15 min/sample and greatly shortened compared with GB 5009.26-2016, and the detection efficiency was increased by 10 times. This method allows rapid detection of N-nitrosamines residues in animal derived foods. 2023 Vol. 37 (3): 33-39 [Abstract] ( 157 ) 全文 ( 278 ) 40 Simultaneous Determination of Eight Antidepressant Residues in Aquatic Products by QuEChERS Purification Combined with High Performance Liquid Chromatography-Tandem Mass Spectrometry KONG Weiheng, ZHENG Zeyang, CHEN Shaobo, CHANG Hong, YANG Fan DOI: 10.7506/rlyj1001-8123-20230110-001 The pollution of antidepressant drugs can lead to their excess residues in aquatic products, which poses a threat to food hygiene and safety. In this study, quick, easy, cheap, effective, rugged and safe (QuEChERS) purification combined with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was used to determine the residues of eight antidepressants, including citalopram, venexaxine, paroxetine, fluoxetine, amitriptiline, tremipramine, sertraline and clomipramine, in common aquatic products such as fish, shrimp, crab and shellfish. The results showed that the linearity of the calibration curves for the eight antidepressants was good in the range of 0.1–5.0 μg/L with correlation coefficients greater than 0.999, the detection limits were 0.04–0.06 μg/kg, and the recoveries of spiked samples were 80.0%–110.0% with relative standard deviation (RSD) of 0.23%–5.50%. This method is simple and rapid and is characterized by low interference, good linearity, and high precision and accuracy. 2023 Vol. 37 (3): 40-45 [Abstract] ( 105 ) 全文 ( 226 ) 46 Development of Ready-to-Use Quality Control Samples for Rapid Detection of Escherichia coli in Meat and Meat Products ZHANG Yalun, CHEN Chen, WANG Hui, ZHANG Tonghe, ZHANG Shuang, LI Xian, LI Yongbo DOI: 10.7506/rlyj1001-8123-20220919-127 This paper developed a ready-to-use quality control sample which can be directly used without secondary culture for the rapid detection of Escherichia coli in meat and meat products by matrix assisted laser desorption/ionization time-offlight mass spectrometry (MALDI-TOF-MS). The sample was evaluated for homogeneity and stability. The results showed that the survival rate of the quality control sample prepared using the selected cryoprotectant was above 90%. The culture count result of the quality control sample (F = 0.567) was less than the critical value, suggesting good uniformity. The quality control sample was stable for 14 days at 37 and 25 ℃, and the resuscitation rates were 101.5% and 99.6% at ?20 and 4 ℃ for 28 days, respectively. After 28 days of storage at 37 and ?20 ℃, the results of MALDI-TOF-MS were consistent, indicating that the developed sample had good stability and met the requirements of quality control samples. 2023 Vol. 37 (3): 46-50 [Abstract] ( 117 ) 全文 ( 198 )        Reviews 51 Progress in the Application of Microfluidics in Meat Quality and Safety Detection CHEN Jiaci, XIAO Bin, ZHONG Ke, CHEN Ailiang DOI: 10.7506/rlyj1001-8123-20221009-133 The protein sources for Chinese people are meat, eggs, milk, soy products, etc., of which the most important are meat and meat products. As a high-protein food, meat is susceptible to microbial spoilage and quality deterioration caused by endogenous enzymes during production, processing, storage and distribution. In addition, the problems of antibiotic and hormone abuse, heavy metal contamination and meat adulteration occur from time to time, which not only harm the vital interests of consumers, but also greatly threaten the personal safety of the public, disrupting the market order and reducing public confidence in food safety. At present, the traditional meat detection techniques are cumbersome and require time-consuming sample pretreatment, so they are unable to achieve fast, cheap and accurate large-scale screening of meat products. In recent years, the development of emerging technologies, especially microfluidics, can well solve the drawbacks of the traditional meat testing methods. This paper analyzes and discusses the concept, history and future of microfluidics and the innovative application of microfluidic devices in meat quality and safety detection, with a view to providing new insights into the development of a new generation of meat testing technology. 2023 Vol. 37 (3): 51-59 [Abstract] ( 149 ) 全文 ( 547 ) 60 A Review of the Working Principle of Electronic Nose Gas Sensor and Its Application in Meat Adulteration Detection XIE Xinru, ZHANG Dequan, LI Shaobo, HOU Chengli, ZHENG Xiaochun, CHEN Li DOI: 10.7506/rlyj1001-8123-20221014-138 As a new bionic olfactory detection technology, the electronic nose has drawn the interest of researchers worldwide for its advantages such as short detection time, simple sample pretreatment, and reliable detection results. At present, there are many new electronic nose sensors based on different detection principles and mechanisms that are available in the market, and the differences in their types and arrays are closely related to the expansion of the application of electronic noses. This paper illuminates the working principles of gas-sensitive sensors used in electronic noses such as metal oxide-type, conductive polymer-type, mass-sensitive, field effect tube-type, and optical fiber-type, analyzes the main factors affecting the results of electronic nose, and summarizes the recent progress in the application of electronic nose technology in the detection of adulteration of fresh meat and meat products, aiming to provide a foundation for the industrial popularization of electronic nose technology and for innovations in meat adulteration detection technology. 2023 Vol. 37 (3): 60-66 [Abstract] ( 2931 ) 全文 ( 419 )

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