Home   |   About the Journal   |   Editorial Board   |   Citation Indices   |   Subscription   |   Open Access Policy   |   Contacts Us   |   中文

Office Online       Submission Online     Peer Review     Editor-in-chief     Office Work Journal Online     Accepted     Current Issue     Advanced Search     Archive     Read Articles     Download Articles     Email Alert

Quick Search     Adv Search 2018 Vol. 32, No. 2 Published: 2018-02-28 Reviews Packaging & Storage Basic Research Analysis & Detection Processing Technology        Basic Research 1 Effect of Dense Phase Carbon Dioxide on Micromorphology of Myosin from Litopenaeus vannamei Muscle LIU Shucheng, DONG Andi, LUO Shuai, CHEN Yali, LIU Yuan, LIU Mengna, JI Hongwu, GAO Jing, MAO Weijie, DENG Chujin, HAO Jiming DOI: 10.7506/rlyj1001-8123-201802001 In the present research, the effect of dense phase carbon dioxide (DPCD) on the micromorphology of myosin extracted from Litopenaeus vannamei muscle was studied by atomic force microscopy (AFM). DPCD treatments were carried out at different pressures and temperatures for different durations. The aggregation characteristics of myosin during the treatments were explored. The results showed that the degree of myosin denaturation and aggregation varied greatly as a function of pressure, temperature and treatment time. DPCD induced myosin denaturation and aggregation by thermally inducing protein-protein interaction as well as by inducing interaction between the protein and carbon dioxide at high pressure. 2018 Vol. 32 (2): 1-7 [Abstract] ( 187 ) 全文 ( 331 ) 8 Antiosteoporotic Effects of Collagen Peptides and Zinc-Chelating Complex from Pearl Oyster Mantle MA Ting, WU Qian, SHEN Xuanri DOI: 10.7506/rlyj1001-8123-201802002 This study was aimed to investigate the effects of peptides (POM-CP) and zinc-chelating peptides (POMCP-Zn) from pearl oyster mantle collagen on the proliferation and differentiation of the osteoblastic cell line MC3T3-E1. The results of methyl thiazolyl tetrazolium assay (MTT) showed that POMCP-Zn significantly stimulated osteoblastic proliferation in comparison to the control, while POM-CP had no effect on cell proliferation. In addition, POM-CP at 400 μg/mL and POMCP-Zn at 10 μg/mL increased alkaline phosphatase activity 1.31- and 1.48-folds in osteoblastic cells compared with the control. Moreover, POMCP-Zn promoted the production of collagen type I and osteocalcin during osteoblastic differentiation and the formation of bone nodules osteoblastic mineralization. In conclusion, POMCP-Zn exerted a stronger effect than POM-CP on the proliferation and differentiation of MC3T3-E1 and POMCP-Zn is a potential candidate functional factor for osteoporosis prevention. 2018 Vol. 32 (2): 8-14 [Abstract] ( 148 ) 全文 ( 284 ) 15 Effect of Wheat and Potato Starches on the Quality of Fish Balls ZHOU Yang, XU Wei, CHEN Jiwang, SUN Wei, XU Chuanwen DOI: 10.7506/rlyj1001-8123-201802003 In this study, wheat and potato starches were added to surimi for the production of fish balls. The gel strength, whiteness and water-holding capacity were tested to measure the quality of fish balls. The results showed that added potato starch had a great effect on the gel strength of fish balls. The gel strength was 1 601 N/cm2 at an addition level of 14%, representing a 255.8% increase compared with the blank control group. The gel strength of fish balls with 10% wheat starch added was 1 498 N/cm2. In addition, The effect of potato starch on the water-holding capacity of fish balls was higher than that of wheat starch. The water-holding capacity increased from 92.31% to 97.80% and from 91.04% to 95.06% with the addition of potato and wheat starches at 14%, respectively. Similarly, potato starch had a stronger effect on the whiteness than did wheat starch. The whiteness decreased with increasing addition of starch. Taking into account their effects on the gel strength and water-holding capacity of fish balls, addition of potato starch at 12% and wheat starch at 10% was appropriate. 2018 Vol. 32 (2): 15-19 [Abstract] ( 208 ) 全文 ( 514 )        Processing Technology 20 Optimization of Rapid Fermentation of Low-Salt Sour Meat by a Combination of Two Lactic Acid Bacteria XIE Yaoyao, YANG Ping, WU Zhanwang, WANG Xiaoyu, HU Ping DOI: 10.7506/rlyj1001-8123-201802004 In this study, two lactic acid bacteria (LAB) strains namely Lactobacillus curvatus SR6 and Pediococcus pentosaceus SR4-2, screened from Dong ethnical sour meat, were used as a mixed starter culture for rapid fermentation of low-salt sour meat. The fermentation process was optimized based on sensory evaluation score and pH value using one-factor-at-a-time method and an L9 (34) orthogonal array design. Quality indicators were measured dynamically during the optimized process in comparison to natural fermentation. The optimal fermentation conditions were obtained as follows: 8% salt, inoculum amount of 2%, 45% glutinous rice and fermentation at 15 ℃. pH value rapidly decreased during the fermentation process and the total amount of free amino acids was significantly increased when compared with natural fermentation (P < 0.05). The optimized process took a shorter period of 30 d to obtain better sour meat quality in terms of improved nutritional quality and safety compared with the traditional process. 2018 Vol. 32 (2): 20-27 [Abstract] ( 214 ) 全文 ( 274 )        Analysis & Detection 28 Simultaneous Determination of 5 Cephalosporins in Fish by High Performance Liquid Chromatography-Tandem Mass Spectrometry BEI Yijiang, WANG Yang, ZHU Ningyu, KE Qingqing, CHEN Xiaofang, LI Shiyan, ZHOU Fan DOI: 10.7506/rlyj1001-8123-201802005 A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was developed for the determination of five cephalosporins in fish (cephalexin, cefalonium, cephapirin, cefquinome and cefazolin). Samples were extracted with acetonitrile and then the extract was cleaned up with a Prime HLB solid-phase extraction (SPE) cartridge prior to being analyzed using an AB-4500 Qtrap LC-MS/MS system. The chromatographic separation was performed on an Accucore C18 RP-MS column (2.1 mm × 100 mm, 2.7 μm) by gradient elution using a mobile phase made up of acetonitrile and 0.1% acetic acid in water at a 0.4 mL/min flow rate. The column temperature was set at 30 ℃. The analytes were detected using multiple-reaction monitoring (MRM) in the positive electrospray ionization mode and quantified by the external standard method. Complete separation was achieved within 8 min. The calibration curves for all five analytes were linear over the concentration range from 0 to 75 μg/kg. The limit of detection for all analytes was 0.5 μg/kg, and the limit of quantification was 2.0 μg/kg. At spiked levels of 10, 25 and 40 μg/kg, the intra-assay recoveries of all five cephalosporins were 81.0%–111.0% with relative standard deviation (RSD) of 0.41%–10.50%, and the inter-assay recoveries were 84.7%– 106.0% with RSD of 0.88%–8.71%. 2018 Vol. 32 (2): 28-32 [Abstract] ( 175 ) 全文 ( 293 ) 33 Surface Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry Analysis of Surface Microbial Differential Proteins in Chilled Tan Sheep Meat HU Qianqian, YANG Bo, LUO Ruiming, YOU Liqin, LI Zixin DOI: 10.7506/rlyj1001-8123-201802006 Microbial proteins on the surface of tray-packaged chilled Tan sheep meat were analyzed by surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and biological samples collected at different times during chilled storage were differentiated. The variable importance in the projection (VIP) value (threshold > 1) of the first principal component of the orthogonal partial least squares-discriminant analysis (OPLS-DA) model was determined and combined with the P value (threshold < 0.05) of student’s t test to find out the differentially expressed proteins. The results showed that 132 differential protein peaks were obtained by Biomarker Wizard software, 75 of which were identified by searching in the Swiss-Prot database based on their mass-to-charge ratio, and the remaining 57 were unknown proteins. These differential proteins did not include enzymes but included 19 proteins that might associated with bacteria on mutton meat. In summary, SELDI-TOF-MS allows the detection of various differentially expressed microbial proteins at different storage times of chilled Tan sheep meat and consequently the identification of a large number of valuable protein markers. 2018 Vol. 32 (2): 33-39 [Abstract] ( 186 ) 全文 ( 290 ) 40 The Authentication of Commercial Fish Products using DNA Barcoding Technique SHI Ruihan, NAN Huizhu, DING Hongtian, WANG Jianchang, FU Qi, SUN Xiaoxia, WANG Jinfeng, DING Lili, CUI Jingfang, LIU Yang, GUO Chunhai DOI: 10.7506/rlyj1001-8123-201802007 This study was conducted to identify the species origin of 91 samples of frozen fish products and highly processed, pre-packaged fish products collected from supermarkets using DNA barcoding technique based on homologous sequence analysis of the COI and 16S rRNA genes. The results confirmed that both COI and 16S rRNA gene sequencing were useful for species authentication of fish products. The species origin of 66 of 79 (83.54%) frozen fish products and 7 of 12 (58.33%) highly processed, pre-packaged fish products was consistent with the product claims, which indicated that some problems such as mislabeling and ambiguous ingredient designation of fish products existed in the fish market. This research can provide technical support for the supervisory authorities concerned . 2018 Vol. 32 (2): 40-45 [Abstract] ( 154 ) 全文 ( 364 )        Packaging & Storage 46 Effect of Chlorine Dioxide on Pomfret Quality during Superchilled Storage JI Xiaotong, NIAN Yiying, XUE Peng, WANG Ling, CUI Yuting, SUN Liming DOI: 10.7506/rlyj1001-8123-201802008 In this paper, pomfret fillet was pretreated with ClO2 and subsequently stored at superchilled temperature (?3 ℃). During storage, meat quality was evaluated by total plate count (TPC), microflora, total volatile basic nitrogen (TVB-N) content, K value, pH value and rheological property (storage modulus) of myofibrillar protein. Results showed that treatment with 30 μg/mL ClO2 (1:20 of solid-to-liquid ratio (m/V), soaking for 10 min) reduced the initial TPC by 2 (lg(CFU/g)). The ClO2 group demonstrated lower TPC than the control throughout the storage period. But during the initial stage, the growth rate of residual bacteria in the ClO2 group was higher than that of the control group. For both groups, the dominant spoilage bacteria were Shewanella and Pseudomonas at the initial and final stages, respectively. According to the TVB-N content, the freshness of the control decreased to second grade after 20 days of storage, whereas that of the ClO2 group remained the same (first grade) throughout the 32-day storage period. ClO2 treatment lowered the pH of fresh fillet by 0.52 and the pH value of the ClO2 group was always 0.2–0.4 lower than that of the control. The K values of the control and ClO2 groups reached 40% on days 12 and 16, respectively, with no significant difference being observed between the two groups during storage. The peak value of storage modulus of myofibrillar protein in the ClO2 group was higher than that of the control during the whole storage period. The above results suggested that the use of ClO2 pretreatment in conjunction with superchilled storage could maintain the freshness and extend the shelf-life of pomfret fillet. 2018 Vol. 32 (2): 46-51 [Abstract] ( 177 ) 全文 ( 287 ) 52 Effect of Carbon Dioxide Anesthesia in Live Tilapia Transportation with Water GUAN Weiliang, CHEN Dewei DOI: 10.7506/rlyj1001-8123-201802009 In this study, hyperoxic carbon dioxide solution was used for relieving the hypoxic stress response of tilapia during simulated transportation. Ammonia excretion was restrained by bubbling carbon dioxide or adding tricaine methanesulfonate (MS-222) into oxygenated water, and the effect was concentration dependent. The longest survival time of tilapia was 548 min in carbon dioxide solution (9 mg/L, pH 6.2) and 710 min in MS-222 solution (20 mg/L), respectively. The survival rate of both groups was 100% in oxygenated water after 4 hours of simulated transportation. In contrast, the survival time of the control group, in which fish were transported with oxygenated water without additive, was 362 min, and the survival rate was only 33%. Therefore, carbon dioxide or MS-222 is an effective sedative for live tilapia transportation. 2018 Vol. 32 (2): 52-56 [Abstract] ( 204 ) 全文 ( 703 )        Reviews 57 Current Situation and Development Strategy of Aquatic Products Processing and Trade in China ZHOU Deqing, YANG Nianqin, WANG Shanshan DOI: 10.7506/rlyj1001-8123-201802010 The import and export trade of aquatic products plays an important role in promoting China’s economic development. China’s aquatic products processing and trade occupy a high proportion of the international market; however, the competitive advantage has diminished in recent years. This paper summarizes the current situation of China’s aquatic products processing and trade, and analyzes the major influencing factors from the perspectives of technical level, cost, the degree of organization of enterprises, processing equipment and international trade barriers. Some development strategies and suggestions are proposed in order to provide a theoretical basis for enhancing China’s competitiveness in aquatic products processing and trade. 2018 Vol. 32 (2): 57-62 [Abstract] ( 183 ) 全文 ( 478 ) 63 Technological Progress and Comprehensive Utilization of Golden Pompano LI Chuan, DUAN Zhenhua DOI: 10.7506/rlyj1001-8123-201802011 Golden pompano is an important marine food source, and its meat is delicious and nutritious. The fish is a good source of fillets and deeply processed surimi products. With the improvement of people’s standards of living, golden pompano has become popular among consumers. Currently, the processing and utilization of golden pompano is still at its infancy in China. In this context, this paper reviews the current status of studies on the nutritional value, frozen processing, preservation and ultra high pressure processing of golden pompano and comprehensive utilization of its byproducts (visceral protease, fish oil and collagen). Through this review, we hope to provide a basis for intensive processing and comprehensive utilization of golden pompano, development of value-added products and effective reduction of environmental pollution, thereby promoting efficient, green and innovative development of golden pompano industry. 2018 Vol. 32 (2): 63-67 [Abstract] ( 191 ) 全文 ( 560 )

News Download Online Submission Author Guidelines Remote Contribute Steps Manuscript Review Process Copyright Statement Submitting Template Ethical Standards Misconduct Processing Links CHINA GENERAL CHAMBER COMMERCE BEIJING CAPITAL AGRIBUSINESS & FOODS GROUP BEIJING ACADEMY OF FOOD SCIENCES CHNFOOD FOOD SCIENCE FOOD SCIENCE AND HUMAN WELLNESS JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY JOURNAL OF FUTURE FOODS

Tel: 010-83155446/47/48/49/50　Fax: 010-83155436　E-mail: meat_research@126.com Powered by Beijing Magtech Co., Ltd.