Simultaneous Determination of 6 β-agonists in Animal-Derived Food by Chemiluminescence Immunoassay
WANG Jianjun1, XIONG Yating2,*, QIN Yu2, YANG Hengfeng3, ZHANG Pengkun4
1.Baoding Animal Products Quality Inspection and Monitoring Center, Baoding 071000, China; 2.Beijing WDWK Biotechnology Co. Ltd., Beijing 100095, China; 3.Hezhou Municipal Bureau of Fishery and Veterinary Medicine, Hezhou 542800, China; 4.Fushun Animal Disease Prevention and Control Center, Fushun 113006, China
Abstract：In this paper, a chemiluminescence immunoassay (CLIA) for simultaneously detecting 6 β-agonist drugs residues in animal-derived food was established. The limits of detection (LODs) for clenbuterol, cimaterol, bromobuterol, bambuterol, mabuterol and sibuterol in pork were 0.48, 1.96, 0.41, 0.29, 0.42 and 0.87 μg/kg, respectively, and in mutton were 0.37, 1.78, 0.43, 0.38, 0.31 and 0.96 μg/kg, respectively. Mean recoveries from spiked samples ranged from 66.7% to 106.4%, and the intra-assay and inter-assay relative standard deviation (RSD) values were less than 15%. The half maximal inhibitory concentration (IC50) of cimaterol was 0.254 μg/L under optimum conditions, and its cross-reaction rates with clenbuterol, bromobuterol, bambuterol, mabuterol and sibuterol were 438.0%, 470.0%, 434.0%, 561.0% and 198.0%, respectively. The developed immunoassay was more accurate and reliable than liquid chromatographic-tandem mass spectrometric (LC-MS/MS). Therefore, chemiluminescence immunoassay proved to be an effective analytical technique for monitoring β-agonist drug residues in animal-derived food owing to its high sensitivity.
2017, Vol. 31 
