Detection of Clenbuterol Hydrochlorid in Swine Urine Using Surface Plasmon Resonance
LIAN Shuangqiu;CUI Dafu;DENG Shaoli
1. Beijing ZhonglongYicheng Technology Co. Ltd., Beijing 100071, China;
2. State Key laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
Abstract：Surface plasmon resonance technique was used to detect clenbuterol hydrochlorid (CLB) in swine urine. clenbuterol hydrochlorid-bovine serum albumin conjugate (CLB-BSA) as a specific recognition probe was bound to the surface of the biological chip, and the solution competition method combined with surface Plasmon resonance technique was used to detect CLB .The calibration curve was established and the limit of detection was 1.03 ng/mL. No cross reaction with ractopamine or salbutamol was found as well as good specificity. The recoveries of CLB from spiked samples were in the range of 98.2；–107.0；, with acceptable coefficients of variation. The proposed SPR method proved to be sensitive, accurate and precise.
2014, Vol. 28 
