Loop-mediated Isothermal Amplification for Detection of Staphylococcus aureus in Porcine Blood
CAI Ke-zhou;XUE Chong-lang;ZHANG Min;WANG Qi;LING Yun-xiao;JIANG Long-fei;JIANG Cheng;JIANG Shao-tong
Key Laboratory for Agriculture Product Processing of Anhui Province, School of Biotechnology and Food Engineering, Hefei
University of Technology, Hefei 230009, China
Abstract：In the present study, a loop-mediated isothermal amplification (LAMP) method was applied to rapidly detect
Staphylococcus aureus in porcine blood samples. The femA sequences of Staphylococcus aureus CMCC10201 were used
as target sequences to design outer primers and inner primers. Amplification products were analyzed by gel electrophoresis.
Eight common pathogenic bacteria were used to test the specificity of the LAMP method, and high specificity to
Staphylococcusaureus was observed, reaching 8.7 CFU/mL. The limit of detection for Staphylococcus aureus in poricine
blood was 8.7 × 102 CFU/mL compared with 8.7 × 103 CFU/mL with PCR. The LAMP method was rapid and highly
specific and sensitive
2012, Vol. 26 
