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| Preparation of Monoclonal Antibody and Fluorescence Immunochromatographic Test Strip for Detecting Furacilin Metabolites |
| GUO Huican, CUI Haibo, XU Dongmei* |
| College of Food and Drug Engineering, Shijiazhuang University of Applied Technology, Shijiazhuang 050000, China |
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Abstract In order to establish a rapid fluorescence immunochromatography method for the detection of semicarbazide hydrochloride (SEM) as a furacilin metabolite, SEM hapten was synthesized and conjugated to bovine serum albumin (BSA) and ovalbumin (OVA) by the active ester method, and the hapten-protein conjugates were employed to obtain anti- SEM monoclonal antibody. Meanwhile, SEM was conjugated to quantumdot microspheres, yielding a probe. Finally, a fluorescence immunochromatography test strip was prepared. The results showed that the detection limit of the test strip for fish meat was 0.247 μg/L. The recoveries of SEM from blank fish samples spiked at different concentrations were between 70% and 120%, and within and between batches coefficients of variation (CV) were below 15%, which was in accordance with the requirements for the determination of veterinary drug residues in animal-derived foods. The test strip was stable at 2–8 ℃ for up to 6 months.
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2019, Vol. 33 
