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| Simultaneous Determination of 11 Quinolone Residues in Livestock and Poultry Meat by QuEChERS and Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry |
| HUANG Kun, WU Wanqin, LUO Tong, FU Wenwen, FAN Xiaolong, MA Yi, WANG Huixia |
| Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Hubei Provincial Institute for Food Supervision and Test, Wuhan 430075, China |
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Abstract A method for simultaneous determination of enrofloxacin, ciprofloxacin, ofloxacin, pefloxacin, norfloxacin, lomefloxacin, sarafloxacin, difloxacin, sparfloxacin, flerofloxacin and danofloxacin residues in livestock and poultry meat by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. Samples were homogenized and extracted with 2% formic acid-acetonitrile. After centrifugation, the supernatant was purified by the QuEChERS procedure, concentrated by a rapid solvent evaporation system, separated on a Waters BEH C18 column (2.1 mm × 50 mm, 1.7 μm) with a mobile phase consisting of 0.1% formic acid-water and 0.1% formic acid-acetonitrile by gradient elution, detected in the multiple reaction monitoring (MRM) mode using an electrospray ionization source (ESI+) and quantified by an internal standard method. The results showed that good linearity was observed for all analytes in the concentration range of 2.0–80.0 ng/mL with correlation coefficients greater than 0.99 and the detection and quantitation limits were respectively 0.5–2.0 and 1.5–6.0 μg/kg. The average recoveries of blank samples spiked at 10, 25 and 50 μg/kg were 74.52%–106.83%, with relative standard deviation (RSD) values of 1.8%–8.7%(n = 6). This method proved to be simple, accurate, highly sensitive and suitable for the simultaneous determination of quinolone residues in livestock and poultry meat.
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2019, Vol. 33 
