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| Establishment of Time-Resolved Fluorescence Immunochromatographic Method for Detection of Abamectin Residues in Yak Meat |
| ZHANG Zhengying, CUI Naiyuan, GAO Haiyan, LIU Haizhen, HUANG Wenying, HAO Yunqing, MA Licai |
| 1.Qinghai Provincial Animal Disease Prevention and Control Center, Xining 810001, China; 2.Beijing WDWK Biotechnology Co. Ltd., Beijing 100095, China; 3.Inner Mongolia Autonomous Region Product Quality Inspection and Research Institute, Hohhot 010020, China |
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Abstract The purpose of this study was to establish and evaluate a time-resolved fluorescence immunochromatographic method for quantitative detection of abamectin residues in yak meat. Anti-avermectin mouse monoclonal antibody G10703 was covalently coupled to carboxylated europium microspheres as a fluorescent marker, which were then sprayed on a release pad, and avermectin antigen and goat anti-mouse antibody were coated on nitrocellulose membrane as the detection line (T) and quality control line (C), respectively to make an immunochromatographic test strip. Finally, a time-resolved fluorescence immunochromatographic method for the detection of abamectin residue in yak meat was established by fitting the four-parameter standard curve between the amount of abamectin added to yak meat samples and the fluorescence signal peak ratio between T-line and C-line (T/C), and its sensitivity, stability, specificity, accuracy and precision were evaluated and compared with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The limit of quantification of this method was 1.0 μg/kg, and the spiked recovery was 86.9%–105.2%. The rates of cross-reactivity with avermectin, ivermectin, doramectin and iprodione were 100.0%, 73.1%, 42.6% and 97.5%, respectively. The within-batch coefficient of variation was 5.4%–9.5%, and the between-batch coefficient of variation was 7.5%–11.2%. The method had high accuracy, precision, sensitivity and stable performance.
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2020, Vol. 34 
