Abstract：The fermentation of chicken blood by Bacillus thuringiensis, Shewanella sp., Penicillium citreonigrum and Aspergillus tubingensis, the dominant bacteria isolated from spontaneous fermentation of chicken blood, for producing liquid fertilizer was optimized using one-factor-at-a-time method and response surface methodology. Free amino acid nitrogen concentration was used as response variable. The optimal conditions obtained from the model developed in this study were as follows: shaking speed 160 r/min, inoculum amount 10%, culture temperature 32 ℃, and culture time 5 d. Under these conditions, the highest free amino acid nitrogen concentration was obtained.
2017, Vol. 31 
