Determination of Clonidine Hydrochloride and Cyproheptadine Hydrochloride in Swine Blood by Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry
YANG Ting，XU Xiuqin, ZHAO Jian, ZHANG Hao, WU Yinliang, WANG Lijun, ZHU Yong
Ningbo Academy of Agricultural Sciences, Ningbo 315040, China
Abstract：A simple and sensitive ultra performance liquid chromatography-tandem mass (UPLC-MS/MS) method for the determination of clonidine hydrochloride and cyproheptadine hydrochloride in swine blood was developed. Samples were extracted with acetonitrile and analyzed by UPLC-MS/MS on a Waters Acquity BEH C18 column with 0.10% formic acid in water/methanol as the mobile phase by means of gradient elution. Detection was carried out with an electrospray ionisation (ESI) probe and operated in the positive ion mode. Clonidine hydrochloride and cyproheptadine hydrochloride was quantified by the external standard method. There was a good linearity in the range of concentrations between 1.0 and 50.0 μg/L. The limits of detection for clonidine hydrochloride and cyproheptadine hydrochloride were both 0.2 μg/L. The recoveries from spiked samples were in the range of 85% to 110% and the relative standard deviations (RSD) were lower than 10%.
