Simultaneous Determination of Six Thiazide-Based Diuretic Residues in Animal Muscle Tissues by High Performance Liquid Chromatography Tandem Mass Spectrometry
ZHENG Kang, XUN Zhiqing, HE Shuang, WANG Chenxia, GUO Yanhua, WU Chusen, HUANG Jinfeng, GUO Xindong
1.Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China; 2.Guangzhou Huaxin Testing Technology Co. Ltd., Guangzhou 510663, China
Abstract：By utilizing solid phase extraction (SPE) and high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS), a simple and rapid analytical method was established for the simultaneous determination of six thiazide-based diuretic residues (chlorothiazide, hydrochlorothiazide, cyclopenthiazide, cyclothiazide, chlortalidone, bendroflumethiazide) in animal muscle tissues. The samples were extracted with acetonitrile-water (30:70, V/V), and the extract was purified with a PRiME HLB solid phase extraction column, separated by reversed-phase high performance liquid chromatography (RP-HPLC), detected with a quadrupole mass spectrometer in the multiple reaction monitoring mode (MRM), and quantified by external standard method. The calibration curves for all analytes showed good linearity in the concentration range of 1–50 μg/L with correlation coefficients higher than 0.997. The limit of quantitation (LOQ) of the method was 1.0 μg/kg. The mean recoveries at three spiked concentration levels were in the range of 82.1%–97.2% with relative standard deviations (RSDs, n = 6) ranging from 3.8% to 9.0%. This method proved to be accurate, simple and rapid and could be applied to detect thiazide-based diuretic residues in animal muscle tissues.
2021, Vol. 35 
