Species-Specific Polymerase Chain Reaction for Authentication of Nototodarus sloanii and Ommastrephes bartramii
YI Jiehan, ZHAO Aihua, WANG Jinrong, GUO Dehui, LI Yanqing, ZHONG Micun
1.Yantai New Era Health Industry Co. Ltd., Yantai 264006, China; 2.Economic Development and Technological Innovation Bureau of Yantai Economic and Technological Development Area, Yantai 264006, China
Abstract：In this study, the mitochondrial cytochrome coxidase subunit Ⅰ (COⅠ ) gene of the two squid species, Nototodarus sloanii and Ommastrephes bartramii, were amplified by polymerase chain reaction (PCR) and sequenced, and DNA polymorphisms were analyzed by multiple sequence alignment. Species-specific primers were designed for each species according to their specific sites, and multiplex PCR was conducted for molecular discrimination of N. sloanii and O. bartramii. As a result, N. sloanii and O. bartramii gave specific amplicons of 214 bp and 339 bp, respectively. The developed species-specific PCR system could detect 1% of intentional adulteration of O. bartramii in N. sloanii with a detection limit of as low as 0.1 ng of genomic DNA. Therefore, it could be useful to identify N. sloanii and O. bartramii and blended surimi from both species available on the market.
2020, Vol. 34 
