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| Transcriptomic Analysis of the Molecular Mechanism of the Effect of Stocking Density on the Quality of Penaeus monodon |
| LI Ling, ZHOU Wei, LI Lu, WANG Yang, SUN Xueliang, LIANG Liya, MA Lizhen |
| 1.College of Food Science and Biological Engineering, Tianjin Agricultural University, Tianjin 300384, China; 2.College of Aquaculture, Tianjin Agricultural University, Tianjin 300384, China |
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Abstract The molecular mechanism of the effect of stocking density on the muscle quality of Penaeus monodon was elucidated by high-throughput transcriptome sequencing and quantitative real time polymerase chain reaction (qRT-PCR). Two stocking densities of 100 and 300 tails/m2 were selected in this study. Among the 6 samples, the minimum values of Q20 and Q30 were more than 90%, and all sequencing data met the requirements of biological information analysis. A total of 778 differentially expressed genes were found between the stocking densities, and their sequences were aligned against seven databases. The differentially expressed genes related to shrimp muscle quality were mainly involved in collagen metabolism, proteolysis, protein transport, cell protein metabolism, cytoskeletal tissues, muscle connectivity, and glucose metabolism. The qRT-PCR results were consistent with transcriptome sequencing.
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2020, Vol. 34 
