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| Preparation and Properties of Fluorescent Immunochromatographic Test Strip for Detection of Phenylethanolamine A |
| GUO Huican1, LI Junhua2, WANG Lijun1, LI Chunsheng2,* |
| 1.College of Food and Drug Engineering, Shijiazhuang University of Applied Technology, Shijiazhuang 050000, China; 2.Biology Institute of Hebei Academy of Sciences, Shijiazhuang 050000, China |
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Abstract A rapid, sensitive and specific immunofluorescence method to detect phenylethanolamine A (PEAA) in urine from farm animals was developed. The diazotization method was used to conjugate PEAA to bovine serum albumin (BSA), yielding an artificial antigen. Furthermore, a specific anti-PEAA monoclonal antibody was obtained by immunization, fusion, and limiting dilution analysis. Finally, a fluorescent immunochromatographic test strip was prepared by coupling quantum dot microspheres with PEAA. The results showed that the detection limit of the test strip was 0.496 μg/L. The recovery was between 80% and 120%, and the inter and intra-batch coefficients of variation (CVs) were both less than 10%. The crossreactivity with other analogues was less than 5%. The specificity was good. Accordingly, the immunofluorescence assay was feasible for the detection of PEAA with good sensitivity, accuracy and specificity and could have wide application prospects.
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2019, Vol. 33 
