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| An Optimized Method for Preparing Nitrosohemoglobin from Pig Blood |
| CHENG Yan;JIANG Wu-bian;FU Shao-hui |
| 1. Hubei Baodi Agri & Tech (Group) Co. Ltd., Anlu 432602, China;
2. Tianjin Baodi Agri & Tech (Group) Co. Ltd., Tianjing 301800, China |
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Abstract Hemoglobin was extracted from the ruptured red blood cells from pigs and then allowed to react with sodium
nitrite to form nitrosohemoglobin. The efficiency of nitrosohemoglobin synthesis as evaluated by OD575 nm was investigated
with respect to four reaction conditions including the amounts of sodium nitrite and vitamin C, pH and heating time using
an orthogonal array design. As a result, a simple, rapid and cost-effective method for preparing nitrosohemoglobin was
established by heating a mixture of hemoglobin, sodium nitrite and vitamin C at a molar ratio of l:2:6 adjusted to pH 6.0 to
60 ℃ and maintaining the temperature for 30 minutes.
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2014, Vol. 28 
