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| Determination of Five Arsenic Species in Aquatic Products by High Performance Liquid Chromatography-Inductively Coupled Plasma Tandem Mass Spectrometry |
| ZHANG Chunlin, GAO Zhuo, WANG Yan, CAO Meng, MA Junmei, ZHANG Leilei, WU Chunmin |
| Hebei Food Safety Key Laboratory, Hebei Food Inspection and Research Institute, Shijiazhuang 050200, China |
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Abstract In this study, an analytical method for the determination of five arsenic species in aquatic products by high performance liquid chromatography-inductively coupled plasma tandem mass spectrometry (HPLC-ICP-MS/MS) was established. In this method, interference was eliminated by means of mass transfer in the MS/MS mode. The mobile phase consisted of 10 mmol/L C2H3NaO2, 3 mmol/L KNO3, and 12 mmol/L NaH2PO4. Gradient elution was used for chromatographic separation on an As7 anion exchange column. Under these conditions, five arsenic species could be completely separated. The developed method exhibited good linearity for trivalent arsenic, dimethyl arsenic, monomethyl arsenic, pentavalent arsenic and arsenic betaine, and the detection limits were 0.005 94, 0.005 28, 0.008 17, 0.008 41, 0.011 70 mg/kg, respectively, which met the analytical requirements. The spiked recoveries ranged between 83% and 116%, and the precision expressed as relative standard deviation (RSD) was less than 5%. This method had the characteristics of simplicity, rapidity, high accuracy, good separation, and high detection efficiency. It could be used to evaluate and analyze various species of arsenic in aquatic products.
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2021, Vol. 35 
