|
|
|
| Effects of Different Temperatures during Waterless Live Transportation on Oxidative Stress in Amur Sturgeon (Acipenser schrencki) |
| ZHOU Wen, BAI Chan, WANG Juguang, CHAI Yi, ZU Xiaoyan, LIAO Tao, XIONG Guangquan |
| 1.Institute of Agro-Products Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; 2.College of Animal Science, Changjiang University, Jingzhou 434023, China |
|
|
|
|
Abstract In order to improve the survival time and survival rate of Amur sturgeon, the fish were anaesthetized with an appropriate concentration of MS-222 to explore the effects of different temperatures (4, 8 and 12 ℃) during waterless live transportation on oxidative stress in it. The survival rate of the fish was recorded after 12 and 24 h of simulated live transportation, and the changes in oxidative stress and antioxidant indicators were analyzed before and after live transportation. The results showed that the appropriate sedative concentration of MS-222 was 100 mg/L and the sedation time was (2.1 ± 0.1) min; the appropriate anaesthetic concentration was 110 mg/L and the anesthesia time was (2.1 ± 0.3) min. After 12 h live transportation, the highest survival rate of 85% was observed at 12 ℃. After 24 h live transportation, the highest survival rate of 62.5% was observed at 4 ℃. After 12 h live transportation, blood sugar, cortisol and malondialdehyde (MDA) levels as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) were significantly lower in the 12 ℃ group than in the other temperature groups (P < 0.05), indicating that the lowest level of oxidative stress occurred in the 12 ℃ group. After 24 h live transportation, there were no significant differences in catalase (CAT) activity among all groups. GPX activity was lower in the 4 ℃ group, and MDA content was significantly lower in this group than in the other groups (P < 0.05). Therefore, when the sturgeon was transported without water for a short time (12 h), a significantly lower level of oxidative stress was observed at higher temperature (12 ℃), suggesting that the appreciate survival temperature was 12 ℃. When the sturgeon was transported for a long time (24 h), low metabolism and anesthesia were maintained better at lower temperature (4 ℃), so that the fish could survive better.
|
|
|
|
|
|
|
|
|
|
|
2021, Vol. 35 
