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| Surface Enhanced Laser Desorption Ionization Time-of-Flight Mass Spectrometry Analysis of Surface Microbial Differential Proteins in Chilled Tan Sheep Meat |
| HU Qianqian, YANG Bo, LUO Ruiming*, YOU Liqin, LI Zixin |
| School of Agriculture, Ningxia University, Yinchuan 750021, China |
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Abstract Microbial proteins on the surface of tray-packaged chilled Tan sheep meat were analyzed by surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS) and biological samples collected at different times during chilled storage were differentiated. The variable importance in the projection (VIP) value (threshold > 1) of the first principal component of the orthogonal partial least squares-discriminant analysis (OPLS-DA) model was determined and combined with the P value (threshold < 0.05) of student’s t test to find out the differentially expressed proteins. The results showed that 132 differential protein peaks were obtained by Biomarker Wizard software, 75 of which were identified by searching in the Swiss-Prot database based on their mass-to-charge ratio, and the remaining 57 were unknown proteins. These differential proteins did not include enzymes but included 19 proteins that might associated with bacteria on mutton meat. In summary, SELDI-TOF-MS allows the detection of various differentially expressed microbial proteins at different storage times of chilled Tan sheep meat and consequently the identification of a large number of valuable protein markers.
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2018, Vol. 32 
