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| Identification of Chicken Origin Ingredients in Livestock and Poultry Meat Based on Mitochondrial DNA COI Gene |
| TANG Xiujun, FAN Yanfeng, GE Qinglian, JIA Xiaoxu, ZHANF Jing, LIU Yinyin, ZHANG Xiaoyan, WANG Jue, GAO Yushi* |
| Institute of Poultry, Chinese Academy of Agricultural Sciences, Yangzhou 225125, China |
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Abstract In this study, a quick method to identify ingredients of chicken origin in livestock and poultry meat was developed by polymerase chain reaction (PCR) and fluorescence quantitative PCR using mitochondrial DNA cytochrome C coxidase subunit Ⅰ (CO Ⅰ ) gene as target gene. The mitochondrial DNA CO Ⅰ genes from chicken, duck, goose, pig, cattle, sheep, rabbit, pigeon and quail revealed distinct loci. Based on this, a set of chicken-specific primers was designed. The results showed that the designed primers could only amplify template DNA from chicken with a cycle threshold (Ct) value of 21.78. The PCR assay had a high specificity and sensitivity (at pg level) and it could be used to detect ingredients of chicken origin in livestock and poultry meat quickly and accurately.
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2017, Vol. 31 
