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| Enzyme-linked Immunosorbent Assay （ELISA） and LC-MS/MS for Determination of Clenbuterol Residue in Animal Tissue |
| WAN Yu-ping，LIU Ning，LI Jin-chao，WANG Shan-liang，NIE Wen-ying |
| 1. Beijing Kwinbon Biotechnology Co. Ltd., Beijing 102206, China；
2. Linyi Xincheng Jinluo Meat Products Co. Ltd., Linyi 276036, China；
3. Hebei Institute of Veterinary Drugs Control, Shijiazhuang 050051, China |
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Abstract Objectives： To compare the precision, accuracy and limit of detection of enzyme-linked immunosorbent assay （ELISA）and liquid chromatography tandem mass spectrometry （LC-MS/MS） in determining clenbuterol residue in animal tissue. Methods： Samples were prepared and then determined by ELISA and LC-MS/MS, respectively. LC-MS/MS was used to confirm positive samples from ELISA analysis. Results： The ELISA recoveries across three spike levels, intra-batch and inter-batch coefficients of variation, sensitivity and limit of detection were 67.0% -- 99.6%, 3.4% -- 8.7%, 6.1% -- 9.6%, 0.025μg/L and 0.025 μg/kg, respectively. For LC-MS/MS, the recoveries across three spike levels were 88.62% -- 111.43%, the intra-batch coefficients of variation 4.4% -- 7.4%, and the limit of detection 0.5 μg/kg. Three positive samples were screened out of 50 pork samples and 50 pig＇s liver samples using ELISA, which was consistent with the results from LC-MS/MS analysis. Conclusion： ELISA is sensitive and accuracy and needs easy sample preparation and low cost, thus being suitable for large-scale screening of clenbuterol residue in animal tissue. In contrast, LC-MS/MS has higher accuracy and can therefore be used to quantify positive samples.
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2011, Vol. 25 
