Abstract：This study is aimed to establish a real time PCR (RT-PCR) method for quantitative determination of porkderived
ingredients in meat and meat products. The pork-specific primers, universal primers and TaqMan probes were
designed for the mitochondrial NADH4 gene and 16S rRNA, respectively, and the established RT-PCR method was
evaluated by specificity, sensitivity, linear relationship and accuracy by using it to detect commercial meat products.
The method showed a good specificity and was able to detect 0.4 pg/μL pork DNA. Both pork-specific and universal
systems showed a good linear relationship (R2 > 0.999) and a wide linear range. The PCR method showed a high
accuracy and the average recovery of pork-derived ingredients in meat mixtures was 122.27%. It can be used to test
both raw and processed pork products.
2013, Vol. 27 
