Abstract：The tenderization of pre-conditioned hot pot beef using a mixture of papain and neutral protease was optimized by one-factor-at-a-time approach and response surface methodology. Mixing ratio between the two enzymes, enzyme dosage and treatment time were considered as independent variables, and water-holding capacity and hardness of beef as responses. It was found that the optimal process conditions were determined as follows: mixing ratio between papain and neutral protease 1:1.19, enzyme dosage 0.085%, and treatment time 3.1 h. Compared with the conventional product, the waterholding capacity of enzymatically tenderized beef increased by 27%, the myofibrillar fragmentation index (MFI) increased by 45%, the solubility of collagen increased by 29%, the shear force decreased by 29%, the hardness dropped by 69%, and the tenderness was significantly improved.
张李智桐，张佳敏，王 卫，刘达玉，唐 杨，王 柯. 响应面法优化预调理火锅牛肉酶法嫩化工艺[J]. 肉类研究, 2022, 36(3): 20-25.
ZHANG Lizhitong, ZHANG Jiamin, WANG Wei, LIU Dayu, TANG Yang, WANG Ke. Optimization of Enzymatic Tenderization of Pre-conditioned Hot Pot Beef by Response Surface Methodology. Meat Research, 2022, 36(3): 20-25.
2022, Vol. 36 
