Establishment of Time-Resolved Fluorescence Immunochromatographic Method for Detection of Abamectin Residues in Yak Meat
ZHANG Zhengying, CUI Naiyuan, GAO Haiyan, LIU Haizhen, HUANG Wenying, HAO Yunqing, MA Licai
1.Qinghai Provincial Animal Disease Prevention and Control Center, Xining 810001, China; 2.Beijing WDWK Biotechnology Co. Ltd., Beijing 100095, China; 3.Inner Mongolia Autonomous Region Product Quality Inspection and Research Institute, Hohhot 010020, China
Abstract：The purpose of this study was to establish and evaluate a time-resolved fluorescence immunochromatographic method for quantitative detection of abamectin residues in yak meat. Anti-avermectin mouse monoclonal antibody G10703 was covalently coupled to carboxylated europium microspheres as a fluorescent marker, which were then sprayed on a release pad, and avermectin antigen and goat anti-mouse antibody were coated on nitrocellulose membrane as the detection line (T) and quality control line (C), respectively to make an immunochromatographic test strip. Finally, a time-resolved fluorescence immunochromatographic method for the detection of abamectin residue in yak meat was established by fitting the four-parameter standard curve between the amount of abamectin added to yak meat samples and the fluorescence signal peak ratio between T-line and C-line (T/C), and its sensitivity, stability, specificity, accuracy and precision were evaluated and compared with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS). The limit of quantification of this method was 1.0 μg/kg, and the spiked recovery was 86.9%–105.2%. The rates of cross-reactivity with avermectin, ivermectin, doramectin and iprodione were 100.0%, 73.1%, 42.6% and 97.5%, respectively. The within-batch coefficient of variation was 5.4%–9.5%, and the between-batch coefficient of variation was 7.5%–11.2%. The method had high accuracy, precision, sensitivity and stable performance.
2020, Vol. 34 
