摘要采用高通量转录组测序和实时定量聚合酶链式反应（quantitative real time polymerase chain reaction，qRT-PCR）技术，在分子水平揭示养殖密度对斑节对虾肌肉品质形成的影响机制。选取养殖密度100 尾/m2、300 尾/m2的斑节对虾为实验材料，进行转录组测序和qRT-PCR验证。结果表明：所有样本Q20和Q30的最小值均大于90%，测序数据均满足生物信息学分析的要求；不同密度养殖条件下，斑节对虾的差异表达基因为778 个；将差异基因序列与7个数据库进行比对，得到不同密度养殖的斑节对虾中与肌肉品质相关的差异基因分布在胶原蛋白代谢、蛋白水解、蛋白转运、细胞蛋白代谢、细胞骨架组织、肌肉连接及葡萄糖代谢途径；qRT-PCR验证结果与转录组测序结果一致。
Abstract：The molecular mechanism of the effect of stocking density on the muscle quality of Penaeus monodon was elucidated by high-throughput transcriptome sequencing and quantitative real time polymerase chain reaction (qRT-PCR). Two stocking densities of 100 and 300 tails/m2 were selected in this study. Among the 6 samples, the minimum values of Q20 and Q30 were more than 90%, and all sequencing data met the requirements of biological information analysis. A total of 778 differentially expressed genes were found between the stocking densities, and their sequences were aligned against seven databases. The differentially expressed genes related to shrimp muscle quality were mainly involved in collagen metabolism, proteolysis, protein transport, cell protein metabolism, cytoskeletal tissues, muscle connectivity, and glucose metabolism. The qRT-PCR results were consistent with transcriptome sequencing.
李 玲，周 伟，李 璐，王 洋，孙学亮，梁丽雅，马俪珍. 基于转录组学的养殖密度影响斑节对虾品质的分子机制[J]. 肉类研究, 2020, 34(12): 1-6.
LI Ling, ZHOU Wei, LI Lu, WANG Yang, SUN Xueliang, LIANG Liya, MA Lizhen. Transcriptomic Analysis of the Molecular Mechanism of the Effect of Stocking Density on the Quality of Penaeus monodon. Meat Research, 2020, 34(12): 1-6.
2020, Vol. 34 
