Abstract：In this paper, liquid chromatography-tandem mass spectrometry (LC-MS/MS) and polymerase chain reaction (PCR) were comparatively used to analyze the animal species of 200 samples of meat skewer sold in the Beijing market. The PCR method was operated in accordance with the entry and exit inspection and quarantine standards, and the LC-MS/MS method was established in our laboratory based on the determined species-specific peptides. The results obtained by the two methods were exactly consistent with each other. The adulteration rate of the meat skewers was 21%, and 28% of lamb skewers and 14% of beef skewers were found to be adulterated. The main adulterants were pig- and duck-derived ingredients. This study further showed that the LC-MS/MS method had the advantages of higher accuracy, easier operations, shorter time consumption, and lower cost than PCR. It can be used for the detection of the animal species of common meat products.
张颖颖，李莹莹，范 维，齐 婧，李慧晨，王守伟. 测定肉串源性成分的液相色谱-串联质谱与聚合酶链式反应方法对比研究[J]. 肉类研究, 2020, 34(7): 70-77.
ZHANG Yingying, LI Yingying, FAN Wei, QI Jing, LI Huichen, WANG Shouwei. Comparison of Liquid Chromatography-Tandem Mass Spectrometry and Polymerase Chain Reaction for Determination of the Animal Species of Meat Skewers. Meat Research, 2020, 34(7): 70-77.
2020, Vol. 34 
