Studies on the Extraction Technique of Superoxide Dismutase from Sheep Blood——The Influence of Thermal Denatureation Temperature and Time and Thermal Change Method on Extraction of SOD,and the Selection of Optimal Technique Condition
YU Ying;XU Guihua
Food Department of Agriculture College of Ningxia University ,Yinchuan 750021, China
Abstract：Objective: To study the effect of thermal denaturation temperature and thermal denaturation time and thermal change method on extraction of SOD, and to define its optimal technique parameter. Method: Orthogonal experiment design was adopted results: From synthetical analysis of the SOD activity and yield productive. The optimal combination of orthogonal experiment is A2B2C2,that is, thethermal denatureation temperature is 55℃;The thermal denatureation time is 15 min; the amount of 0.01mol/l CuCl2 addition is 2%. Conclusion: Thermal denaturation is one of the most importance in extracting SOD from sheep blood, higher purity SOD, whose activity exceeds 1 000 U/mg protein was reached by this technique.
于颖；徐桂花. 羊血中超氧化物歧化酶提取工艺研究——热变温度、热变时间和热变方式对SOD提取的影响及最佳工艺条件选择[J]. 肉类研究, 2009, 23(10): 32-35.
YU Ying;XU Guihua. Studies on the Extraction Technique of Superoxide Dismutase from Sheep Blood——The Influence of Thermal Denatureation Temperature and Time and Thermal Change Method on Extraction of SOD,and the Selection of Optimal Technique Condition. Meat Research, 2009, 23(10): 32-35.
2009, Vol. 23 
