Study on the Heating Denatureation Extraction of SOD from Sheep Blood with Response Surface Analysis
YU Ying;WANG Xiaoli;XU Guihua
1.Food Department of Agriculture College of Ningxia University,Yinchuan,750021,China;2.Ningxia Agricultural Reclamation Management Bureau,Yinchuan,750001,China
Abstract:Based on single factor tests,the optimum heating denatureation extraction conditions of SOD from sheep blood were obtained through Box-benhnken central combination design and RSM.The results showed that the optimum heating denatureation extraction conditions of SOD are as follows:heating denatureation temperature 55℃,heating denatureation time 15min and 2%.Under such conditions,the activity exceeds 3 000 U/mg protein was reached.
于颖;王小丽;徐桂花. 响应面分析法优化羊血中SOD的热变性提纯工艺条件[J]. 肉类研究, 2009, 23(11): 25-30.
YU Ying;WANG Xiaoli;XU Guihua. Study on the Heating Denatureation Extraction of SOD from Sheep Blood with Response Surface Analysis. Meat Research, 2009, 23(11): 25-30.