Abstract:Sheep’s cardiac muscle was used as raw material to preparate cytochrome C.The yield rate of cytochrome C as index,the extraction results of extraction solution with different pH values,different extraction temperature and trichloroacetic acid with different dosage were compared.The results showed that the optimal extraction conditions were as follows: pH of acidified water 3.6,extraction temperature 35℃ sedimentation by 22ml/L of tricholoracetic acid with concentration of 20%.Under these conditions,the yield rate of cytochrome C was 232.6 mg/kg.
于颖;徐桂花. 羊心脏中细胞色素C的提取研究[J]. 肉类研究, 2009, 23(4): 31-34.
YU Ying;XU Guihua. Study on the Extraction of Cytochrome C from Sheep Heart. Meat Research, 2009, 23(4): 31-34.