Determination of Residual Ofloxacin Enantiomers in Chicken Muscle by Liquid Chromatography
YE Yu-fei;LIU Yong-jun;ZHAO Jian;L Yan;WU Yin-liang
1. The Ningbo Academy of Agricultural Sciences, Ningbo 315040, China;
2. The Center for Animal Disease Control and Prevention, P.R. China, Beijing 100125, China
Abstract：A method was developed for determining residual ofloxacin enantiomers in chicken muscle by liquid chromatography with fluorescence detection. Samples were extracted with phosphate buffer solution. The extract was purified by solid phase extraction (SPE) using a C18 cartridge and the eluent was then dried by nitrogen blowing and dissolved in mobile phase. The analytes were separated on a LeapsilTM C18 column using a mobile phase composed of a mixture of methanol and aqueous solution of (pH 3.5) 2 mmol/L copper sulfate and 2.5 mmol/L isoleucine. Excitation wavelength was 297 nm and emission wavelength was 487 nm. Quantitation was performed by using the external standard calibration method. The average recoveries for the two enantiomers in spiked chicken muscle were 75.5%-86.1%at four fortiifed levels ranging from 5 to 150μg/kg, and the intra-and inter-batch coefifcients of variation (CVs) were 2.15%-4.21%and 3.87%-5.84%, respectively. The limits of detection (LOD) and quantitation (LOQ) were 1.5 and 5.0μg/kg for the two enantiomers of olfoxacin, respectively.
2014, Vol. 28 
