Advances in Meat Adulteration Detection Based on CRISPR/Cas Technology
GUO Runze, ZHANG Ying, YANG Ruiqin, SUN Qifan
1. School of Investigation, People’s Public Security University of China, Beijing 100038, China; 2. Institute of Forensic Science, Ministry of Public Security, Beijing 100038, China
Abstract：In recent years, meat adulteration has become a prominent issue threatening food safety and touching on religion, economy, and more, making it a pressing social problem. While various detection methods exist for meat adulteration, traditional techniques fall short in meeting the demand for rapid and accurate on-site detection. Thus, developing rapid and accurate methods for on-site detection of meat adulteration is of great significance. The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) system, composed of CRISPR and Cas, has the advantages of trans-cleavage activity and high specificity and has become an ultrasensitive nucleic acid detection tool. It shows significant potential in meat adulteration detection. This paper reviews the pros and cons of the existing methods for meat adulteration detection, and introduces the composition, classification, and mechanism of action of the CRISPR/Cas system, with a focus on the application of CRISPR/Cas-based technology in meat adulteration detection. It also discusses the advantages, challenges and future directions of this technology.
2026, Vol. 40 
