Comparative Studies on Enzymatic and Acidic Extraction and Properties of Collagen from Crocodile Skin
BAO Yu-long;CHEN Sun-fu;LUO Yong-kang
1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
2. Guangxi Mengzhan of the Crocodile Science and Technology Development Co. Ltd., Nanning 530000, China
Abstract：Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from the skin of crocodile(Crocodylus )
were prepared and comparatively characterized. Both collagens displayed a distinct absorption peak at 232 nm in their
UV absorption spectra. SDS-PAGE analysis showed that the peptide chain composition of ASC and PSC revealed a high
similarity, both of which contained α 1,α 2, β and γ chains. The solubility test results suggested that the isoelectric point of
collagen from crocodile skin was around pH 7. The water-holding capacity of ASC and PSC remained more than 85% after
heat treatment for 6 h at 25 ℃. A considerable difference in oil-binding capacity was found between ASC (24 mL/g) and
PSC (41 mL/g). From the above results, it can be concluded that both collagens from crocodile skin have similar structures
and the characteristics of collagen type I despite their differences in functionality.
包玉龙；陈孙福；罗永康. 酸法和酶法提取鳄鱼皮胶原蛋白及性质研究[J]. 肉类研究, 2012, 26(7): 1-4.
BAO Yu-long;CHEN Sun-fu;LUO Yong-kang. Comparative Studies on Enzymatic and Acidic Extraction and Properties of Collagen from Crocodile Skin. Meat Research, 2012, 26(7): 1-4.
2012, Vol. 26 
