A Dual Fluorescent Real-Time Polymerase Chain Reaction Method for Concurrently Detecting Pork-Derived and Chicken-Derived Ingredients in Animal-Origin Foods
JIN Ping, JIE Li, LU Jun, CHEN Ying, DING Hongliu*
Suzhou Quality Supervision and Inspection Center, Suzhou 215128, China
Abstract：Objective: To establish a dual fluorescent real-time polymerase chain reaction (PCR) assay for the simultaneous
detection of pork-derived and chicken-derived ingredients which enables rapid detection of adulteration in animalorigin
foods. Methods: With this aim, specific primers and TaqMan probes were designed according to the interspecific
conservative sequences. Results: The dual fluorescence PCR method had good specificity and could only amplify porkderived
ingredients and chicken-derived ingredients. The method had high sensitivity and could detect as low as 0.02 ng
of porcine DNA and 0.10 ng of chicken DNA. It had strong anti-interference ability and could correctly detect more than
2% pork-derived and chicken-derived ingredients spiked in mixed DNA samples with other species. Conclusion: The dual
fluorescent real-time PCR provides a specific and sensitive method for the detection of pork and chicken-derived ingredients
in animal-origin foods.
