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| The Study of the Immune Effect of Extraction and Purification of IgM from the Mucus of Catfish |
| 1. College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang, 150030；2. Department of Food Science,Tianjin Agricultural University, Tianjin, 300384) |
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Abstract Abstract: This experiment extract and purify IgM from the mucus of catfish by means of gradient sediment using ammonium sulfate and ion-exchanged Chromatography.The results showed that the best pH for ion-exchanged chromatography was 7.4, the best velocity was 3ml/min, and the concentration of NaCl was 1mol/L.The relative molecular mass of the heavy chaine and light chain of IgM was 78ku and 26ku,respectively,as determined by SDS-PAGE.Trough the experiment of rat Splenic lymphoid cell proliferation, we know that when the concentration of IgM was 6 mg/mL, its immunoregulatory activity was better.
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2010, Vol. 24 
