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| Determination of Furazolidone Metabolite Residue in Pork by Enzyme-linked Immunosorbent Assay |
| MIN Cheng-jun1，LUO Xiao-qin2,*，WANG Shan-liang2，CUI Ting-ting2 |
| 1. Yurun Group Co. Ltd., Nanjing 210041, China；2. Beijing Kwinbon Biotechnology Co. Ltd., Beijing 102206, China |
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Abstract An enzyme-linked immunosorbent assay （ELISA） was proposed to determine residual furazolidone metabolite in pork using a commercially available ELISA kit. The results showed that the sensitivity and detection limit of the assay were 0.025-- 2.025 μg/L and 0.1 μg/kg, respectively. The recoveries of standard additions of furazolidone metabolite averaged 78.2% -- 100.3% across three levels （n = 3）, and the coefficients of variation were 4.7%--10.3%. The cross-reactivity towards furazolidone was 16.3%; as for other drags investigated, the cross-reactivity was below 1%. Analytical results obtained using the assay were in substantial accordance with those from liquid chromatographic tandem mass spectrometry （LC-MS/MS） determination. The assay was sensitive, accurate, repeatable, specific and suitable for the determination of furazolidone metabolite residue in pork.
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2011, Vol. 25 
