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| An Optimized CTAB Method for Total RNA Extraction from Meat |
| ZHOU Tong，LI Jia-peng，TIAN Han-you，YANG Jun-na，QIAO Xiao-ling |
| China Meat Research Center, Beijing 100068, China |
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Abstract An optimized CTAB method for total RNA extraction was presented for use in accurately quantifying components of animal origin and fighting against adulterated food in the market as a sample preparation method. The use of the CTAB method to extract total RNA from pork was investigated with respect to sample amount, dissociation time, precipitation time and RNase treatment. As a result, a fast and efficient method for total RNA extraction was achieved by selecting sample amount below 0.05 g, 0.5 h dissociation, 0.5h precipitation and non-RNase treatment. The method showed a good linear relationship （R2 = 0.9972） and stability （SD = 0.18）.
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2011, Vol. 25 
