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| Comparative Studies on Enzymatic and Acidic Extraction and Properties of Collagen from Crocodile Skin |
| BAO Yu-long;CHEN Sun-fu;LUO Yong-kang |
| 1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China；
2. Guangxi Mengzhan of the Crocodile Science and Technology Development Co. Ltd., Nanning 530000, China |
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Abstract Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) from the skin of crocodile(Crocodylus )
were prepared and comparatively characterized. Both collagens displayed a distinct absorption peak at 232 nm in their
UV absorption spectra. SDS-PAGE analysis showed that the peptide chain composition of ASC and PSC revealed a high
similarity, both of which contained α 1,α 2, β and γ chains. The solubility test results suggested that the isoelectric point of
collagen from crocodile skin was around pH 7. The water-holding capacity of ASC and PSC remained more than 85% after
heat treatment for 6 h at 25 ℃. A considerable difference in oil-binding capacity was found between ASC (24 mL/g) and
PSC (41 mL/g). From the above results, it can be concluded that both collagens from crocodile skin have similar structures
and the characteristics of collagen type I despite their differences in functionality.
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2012, Vol. 26 
