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| Optimization of Extraction Process for Acid Soluble Collagen from Jumbo Squid (Dosidicus gigas) Skin and Evaluation of Its Characteristics |
| LIU Meng |
| China Meat Food Research Center, Beijing 100068, China |
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Abstract The extraction of acid soluble collagen (ASC) from jumbo squid skin was optimized using one-factor-at-a-time method and response surface methodology (RSM). Physiochemical properties of ASC were analyzed. The results demonstrated that the optimum extraction conditions that provided the maximum yield of ASC (31.76%) were determined as 4 ℃, 0.47 mol/L, 24.5 mL/g and 43 h for temperature, acetic acid concentration solvent-to-solid ratio and extraction time, respectively. The results of UV full wavelength scanning indicated that ASC had a maximum absorption peak at 231 nm wavelength, which was typical of type-Ⅰ collagen. In addition, the result of gel electrophoresis showed ASC contained α1, α2 and β chains. Moreover, Raman spectral analysis illustrated that the structural integrity of ASC was intact without any damage to its triple helical structure. The phase transition temperature of ASC was determined to be 50.07 ℃.
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2020, Vol. 34 
