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| Detection of Ceftriaxone Sodium Residue in Livestock and Poultry Meat by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| HUANG Meiling |
| GRG Metrology and Test Fuzhou Co. Ltd., Fuzhou 350001, China |
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Abstract In this study, an analytical method for the determination of ceftriaxone sodium residues in meat using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples were homogenized with acetonitrile-water (8:2, V/V) using a vortex mixer and ultrasonically extracted, and the extract was cleaned up by the quick, easy, cheap, effective, rugged and safe (QuEChERS) method, and separated on a BEH C18 column using a mobile phase consisting of 0.1% formic acid and methanol. The analysis was performed under multiple reaction monitoring (MRM) in the positive electrospray ionization (ESI+) mode. The external standard method was used for quantification. The results showed that the calibration curve for ceftriaxone sodium had good linearity, with a correlation coefficient greater than 0.999. The detection limit was 3.0 μg/kg, and the quantification limit was 9.0 μg/kg. The average recoveries for different matrices spiked at three concentration levels were 87.17%–105.21% with relative standard deviations (RSDs) ranging from 3.14% to 6.13%. This method is simple, rapid, accurate, sensitive, highly operable, and suitable for the rapid detection of ceftriaxone sodium in meat.
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2023, Vol. 37 
