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| Detection of Ceftriaxone Sodium Residue in Livestock and Poultry Meat by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry |
| HUANG Meiling |
| GRG Metrology and Test Fuzhou Co. Ltd., Fuzhou 350001, China |
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Abstract In this study, an analytical method for the determination of ceftriaxone sodium residues in meat using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. The samples were homogenized with acetonitrile-water (8:2, V/V) using a vortex mixer and ultrasonically extracted, and the extract was cleaned up by the quick, easy, cheap, effective, rugged and safe (QuEChERS) method, and separated on a BEH C18 column using a mobile phase consisting of 0.1% formic acid and methanol. The analysis was performed under multiple reaction monitoring (MRM) in the positive electrospray ionization (ESI+) mode. The external standard method was used for quantification. The results showed that the calibration curve for ceftriaxone sodium had good linearity, with a correlation coefficient greater than 0.999. The detection limit was 3.0 μg/kg, and the quantification limit was 9.0 μg/kg. The average recoveries for different matrices spiked at three concentration levels were 87.17%–105.21% with relative standard deviations (RSDs) ranging from 3.14% to 6.13%. This method is simple, rapid, accurate, sensitive, highly operable, and suitable for the rapid detection of ceftriaxone sodium in meat.
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ZHAN Hao, XUAN Lei, KONG Weiheng, LIU Wen, CHEN Ranran, MENG Xiaodi, ZHOU Xiaoying, Lü Yaning, HAN Fang, YU Xiaofeng. Rapid Determination of 12 Diuretic Residues in Pork by Modified QuEChERS Combined with Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Meat Research, 2025, 39(12): 69-75. |
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2023, Vol. 37 
