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| Establishment of a High Performance Capillary Electrophoresis Method for the Determination of Amoxicillin and Ampicillin Residues in Freshwater Fish |
| SUN Xuefeng, WANG Fangyu |
| Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450000, China |
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Abstract This study established a high-throughput method for the simultaneous and efficient detection of amoxicillin and ampicillin residues in silver carp as a representative of freshwater fish using high performance capillary electrophoresis (HPCE) with a diode array detector. Samples were extracted with acetonitrile, concentrated by rotary evaporation and cleaned up using a solid phase extraction (SPE) column. The capillary column was washed with 30 mmol/L borax buffer solution at pH 7.85. The detection wavelength, voltage and temperature were set at 210 nm, 20 kV and 25 ℃, respectively. Baseline separation of amoxicillin and ampicillin was accomplished within 20 min. The peak area of amoxicillin and ampicillin showed a linear correlation with their concentrations in the range of 0.02–10.00 μg/mL, with correlation coefficients (R2) greater than 0.990. The recoveries of amoxicillin and ampicillin spiked in freshwater fish were 78.90%–85.72% and 75.36%-86.34%, respectively, with within-day coefficient of variation (CV) less than 8.01% and 8.06%, respectively.
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2023, Vol. 37 
