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| Establishment of a High Performance Capillary Electrophoresis Method for the Determination of Amoxicillin and Ampicillin Residues in Freshwater Fish |
| SUN Xuefeng, WANG Fangyu |
| Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou 450000, China |
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Abstract This study established a high-throughput method for the simultaneous and efficient detection of amoxicillin and ampicillin residues in silver carp as a representative of freshwater fish using high performance capillary electrophoresis (HPCE) with a diode array detector. Samples were extracted with acetonitrile, concentrated by rotary evaporation and cleaned up using a solid phase extraction (SPE) column. The capillary column was washed with 30 mmol/L borax buffer solution at pH 7.85. The detection wavelength, voltage and temperature were set at 210 nm, 20 kV and 25 ℃, respectively. Baseline separation of amoxicillin and ampicillin was accomplished within 20 min. The peak area of amoxicillin and ampicillin showed a linear correlation with their concentrations in the range of 0.02–10.00 μg/mL, with correlation coefficients (R2) greater than 0.990. The recoveries of amoxicillin and ampicillin spiked in freshwater fish were 78.90%–85.72% and 75.36%-86.34%, respectively, with within-day coefficient of variation (CV) less than 8.01% and 8.06%, respectively.
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LING Yihan, WANG Xiaomeng, WU Shouxun, ZHOU Ting, LI Xingjie, ZHOU Heting, ZHANG Shuwen, YIN Wenya, WANG Jianping, WANG Yi. Determination of Residues of the Novel Veterinary Drug Fluralaner in Chicken Tissues and Eggs by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Meat Research, 2024, 38(10): 30-35. |
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2023, Vol. 37 
