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| Determination and Uncertainty Evaluation of Vancomycin Residues in Meat by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry |
| LI Huichen, ZHANG Yingying, GUO Wenping, SONG Yongqing, FAN Zhicong |
| Beijing Key Laboratory of Meat Processing Technology, China Meat Research Center, Beijing 100068, China |
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Abstract An ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed for the determination of vancomycin residues in meat samples. Sample were extracted with a mixed solution of formic acid and acetonitrile, degreased with n-hexane and purified by solid-phase extraction on C18 cartridges. The target compound was detected by electrospray ionization (ESI) in positive ion mode with multiple reaction monitoring (MRM) and quantified by an external standard method. The results showed a good linear relationship was found between the peak area of quantitative ions and the concentration of vancomycin with a correlation coefficient of 0.999 7, and the limit of detection (LOD) of this method was 0.1 μg/kg. The recoveries of vancomycin at three different spiked levels ranged from 86.7% to 100.3%, with a relative standard deviation (RSD) of 2.52%. In addition, the uncertainty for the determination of vancomycin residues in meat using this method was evaluated. It was found that at the 95% confidence level, the residual amount of vancomycin in pork was expressed as (0.342 ± 0.041) μg/kg when the expansion factor (k) was 2. The proposed method was simple, efficient, sensitive, accurate, stable, and suitable for routine analysis.
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2022, Vol. 36 
