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| Determination of Amantadine in Chicken Muscle by Liquid Chromatography with Tandem Mass Spectrometry Coupled with Dispersive Solid Phase Extraction Using Multiwalled Carbon Nanotubes as Adsorbent |
| SUN Ya-mi;LIU Yong-jun;ZHAO Jian;YE Yu-fei;L(U) Yan;WU Yin-liang |
| 1. Ningbo Academy of Agricultural Sciences, Ningbo 315040, China;
2. The Center for Animal Disease Control and Prevention, P.R. China, Beijing 100125, China |
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Abstract A simple, sensitive and reliable analytical method was developed for amantadine in chicken muscle by ultra high
performance liquid chromatography-positive electrospray ionisation tandem mass spectrometry (UHPLC-ESI-MS/MS) using
dispersive solid phase extraction (dSPE) with multi-walled carbon nanotubes (MWCNTs). Chicken muscle was extracted
using 1.0% acetic acid in acetonitrile. After adjusting pH value to 11, 75 mg of MWCNTs were added to absorpt amantadine
and 5.0% formic acid solution:methanol (5:5, V/V) was used to elute the compound from the MWCNTs. The eluate was
directly analyzed by LC-MS-MS on an Acquity UPLC BEH C18 column with a mixture of methanol and 0.1% formic
acid solution as the mobile phase. The samples were quantified with the internal standard calibration curve method. Good
linearities were obtained for amantadine in the concentration range of 0.05–5.0 μg/L with correlation coefficient more than
0.999. The recoveries for amantadine in chicken muscle were 97.8%–103.6% at three fortified levels (0.5, 0.75 and 1.0 μg/kg)
with relative standard deviations less than 10.0% (n = 3). The limit of quantitation was 0.5 μg/kg.
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2014, Vol. 28 
