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Study on the reaction condition of the heme extraction of the porcine blood |
Zhao Jin-song |
Sichan University of science & Engineering, zigong, Sichuan, 64300, china |
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Abstract extracting heme form pig blood was studied in this paper. It was used orthogonal experiment to investigate the best conditions of extraction techniques for heme, that is: dissolve the blood with three times clean water, four times chloroform; then add six times acetone in it when setting it apart, and regulate the pH to 3; and a time 1mol/L sodium acetate in when heme crystallizing, regulate the pH to 5.according to this, it can be accquined about 4.9201g heme with purity at 60% form 1000ml pig blood.
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