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| Optimization of Iron Chelating for Pig Blood Derived Composite Iron Supplement Containing Multiple Trace Elements by Response Surface Methodology |
| HUANG Jingjing, ZHANG Fusheng, YAN Yan, YIN Junfeng, ZHOU Yingqin, XIE Ningning* |
| Institute of Agricultural Products Processing, Anhui Academy of Agricultural Sciences, Hefei 230031, China |
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Abstract The objective of this study was to optimize the chelating process for composite microelement iron supplement prepared from pig blood by response surface methodology. By using one-factor-at-a-time method, pH value, peptide concentration, and volume ratio of peptide solution to FeCl2 solution (1.0 mol/L) were identified as factors that significantly influence Fe2+ chelating efficiency. Subsequently, three-dimensional response surface plots were generated using a threevariable three-level Box-Behnken design to investigate the interaction among the three factors. The optimized conditions were established as follows: pH 5.40, peptide concentration 2.27%, and volume ratio of peptide solution to FeCl2 solution (1.0 mol/L) 2.16:1. Under these conditions, the predicted Fe2+ chelating efficiency was 79.37%, and the actual data was 79.41%.
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2018, Vol. 32 
