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A Review of Recent Advances in the Application of DNA Barcoding in Identification of Animal Species in Highly Processed Animal-Derived Products |
LING Shengnan1,2, WU Yajun1, HAN Jianxun1, QIAO Xuguang2, CHEN Ying1,* |
1.Agro-product Safety Research Center, Chinese Academy of Inspection and Quarantine, Beijing 100123, China;
2.College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China |
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Abstract DNA barcoding is considered to be a powerful, non-directional, specific, accurate and economical method that can
be applied widely. As a technique of great interest for researchers to identify special origin in the field of molecular biology,
DNA barcoding amplifies specific DNA sequences by using universal primers, which helps to overcome the shortcomings
of the traditional detection methods and transcend the limitation of many molecular methods to directional detection.
DNA barcoding nowadays has been widely applied in the fields of environmental and ecological protection, medicine and
healthcare, and food safety. This paper outlines the basic principle, developmental process and current studies of DNA
barcoding and summarizes its application in the detection of animal species in highly processed animal-derived products
like animal-derived traditional Chinese medicinal materials, marine animal products, meat and meat products, animalderived
health products, leather products, and animal specimens. Finally we conclude this review with the advantages and
disadvantages of DNA barcoding as well as some perspectives on the future development trend of DNA barcoding.
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