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| Determination of Residual Clenbuterol Enantiomers in Pork by Liquid Chromatography with Tandem Mass Spectrometry |
| CHEN Guo1, LIU Yongjun2, Lü Yan1, SUN Yami1, WU Yinliang1,* |
1. The Ningbo Academy of Agricultural Sciences, Ningbo 315040, China;
2. The Center for Animal Disease Control and Prevention of China, Beijing 100125, China |
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Abstract A method was developed for determining residual clenbuterol enantiomers in pork by liquid chromatography with tandem mass spectrometry. Five grams of samples were extracted with ethyl acetate under basic condition. Then, clenbuterol enantiomers were back-extracted from the extracts using diluted hydrochloric acid. The hydrochloric acid extract was purified by SCX solid phase extraction (SPE) cartridge. The eluent was dried by blowing nitrogen and the residue was dissolved in 500 μL of methanol with 0.02% acetic acid and 0.1% ammonium acetate. The analytes were analyzed by LC-MS/MS on a chiral column (Astec CHIOBIOTICTM V) with a mixture of methanol-acetic acid-ammonium acetate as the mobile phase and quantified by the internal standard calibration curve method. Good linearities were obtained for two enantiomers in the concentration range of 2.0 – 200 μg/L with a correlation coefficient more than 0.997 0. The recoveries for the two enantiomers in pork were 95.8% – 102.1% at the fortified levels of 0.25 – 0.75 μg/kg with relative standard deviations less than 5%. The limits of detection (LOD) and quantitation (LOQ)were 0.10 and 0.25 μg/kg, respectively.
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| [1] |
WANG Yanli, LIANG Xiuqing, CHEN Qianqian, LI Fangfang, LI Jie, CHEN Keyun, TIAN Qiyan, LI Xia, LIU Yanming. Determination of Eleven N-Nitrosamines in Animal Derived Foods by Gas Chromatography-Tandem Mass Spectrometry after Pass-Through Solid Phase Extraction[J]. Meat Research, 2023, 37(3): 33-39. |
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