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| Optimization of Conditions for Collagen Extraction with Lactic Acid from Channel Catfish （Ictalurus punctatus） Skin |
| GONG Zi-hui，LIN Lin，MENG Chang-wei，YE Ying-wang，JIANG Shao-tong，LU Jian-feng |
| College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China |
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Abstract This study was undertaken to optimize the acidic extraction of collagen from channel catfish （lctalurus punctatus） skin. Lactic acid was found to be a better solvent for extracting collagen from channel catfish skin than formic acid, acetic acid, citric acid and hydrochloric acid. Further, three process conditions affecting the extraction rate of collagen with lactic acid, such as pH value, solid-to-liquid ratio and extraction time, were optimized by one-factor-at-a-time method and Box-Benhnken experimental design combined with response surface methodology. The results indicated that the optimal conditions for collagen extraction were pH 2.3, solid-to-liquid ratio 1：60 （g/mL） and extraction time 46 h, under which, an extraction rate of 46.28% was obtained.
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2011, Vol. 25 
