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| Effect of Different Sterilization Treatments on Heparin Titer, Protein Content in Wastewater and Microflora in Porcine Small Intestine |
| QUAN Run, WU Xiaolong, CHONG Zhengchen, LIU Rongxu, ZHANG Junjie, WANG Yucong, XIE Zhixin, HAN Jianchun, LIU Danyi |
| 1. College of Food Science, Northeast Agricultural University, Harbin 150030, China; 2. Heilongjiang Institute of Green Food Science, Harbin 150028, China; 3. Heilongjiang Hanheng Environmental Protection Technology Co. Ltd., Harbin 150000, China |
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Abstract To reduce microbial contamination during heparin extraction from porcine small intestines, this study employed slightly acidic electrolyzed water (SAEW). The sterilization effects of three SAEW treatments: direct immersion, ultrasonicassisted immersion and spraying were evaluated as well as their influences on the bacterial reduction rate, heparin titer and the protein content of wastewater to screen the best sterilization treatment. 16S rRNA high-throughput sequencing was used to analyze the composition and changes of microflora in porcine small intestine after the best sterilization treatment. The results showed that under optimal conditions, there was no significant difference in the sterilization effect of the three methods. Of these, spray treatment had the least effect on the heparin titer and the protein content of wastewater, and was thus selected for sterilization before heparin extraction. High-throughput 16S rRNA gene sequencing revealed that spraying SAEW to an available chlorine concentration of 75 mg/L killed the dominant genera in the porcine small intestine, such as Lactococcus, Vagococcus and Psychrobacter, reducing the microbial contamination of porcine small intestinal heparin.
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2025, Vol. 39 
