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| Development of DNA Detection Kit for Dog, Fox and Mink-Derived Components in Adulterated Meat Products |
| JIA Huijian, WANG Tiantian, ZHAO Yuan, SONG Shunjia, SHAO Xuechao, AI Jinxia, SUN Liyuan |
| School of Medical Technology, Beihua University, Jilin 132013, China |
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Abstract In order to rapidly detect DNA derived from the common Canidae animals dog, fox and mink in adulterated meat products, molecular biology technology was applied to analyze the differences in the genomic sequence of various species, and a multiplex polymerase chain reaction system was establish and optimized to develop rapid detection kits. The results showed that the developed kit had good specificity without cross-reaction with common food-producing animal-derived DNA components (pigs, cattle, sheep, horses, donkeys, chickens and ducks), as well as high sensitivity. When the DNA concentration of the three target samples decreased to 10-4 ng/μL at the same time, they still could be accurately detected using the kit. The positive bands were excised, cloned and sequenced, showing 100% similarity to the dog (MH105046.1), fox (LT560065.1) and mink (KU145464.1) sequences deposited in the GenBank database. This kit correctly identified 30 simulated mixed meat samples with 100% accuracy. Of 50 commercially available samples, three dog meat samples were found to be adulterated.
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2021, Vol. 35 
